Object Details
- Yanagimachi, Ryuzo
- Wakayama, Teruhiko
- Description
- These are the remains of a mouse [born October 3, 1997; died May 5, 2000] that was cloned from an adult somatic cell by Prof. Ryuzo Yanagimachi and Dr. Teruhiko Wakayama at the University of Hawai'i, Mānoa. Wakayama was a post-doctoral researcher in Yanagimachi's lab when he started cloning experiments. This mouse was the second animal cloned from an adult somatic cell. The first, a Finn Dorset ewe named Dolly, was created at the Roslin Institute in Scotland in 1996.
- Every cell in an animal other than its reproductive cells are somatic cells. The somatic cell chosen to create this mouse was a cumulus cell, which help eggs mature. The mouse was subsequently named Cumulina. Wakayama and Yanagimachi removed the nucleus from the cumulus cell of an agouti mouse, inserted it into an enucleated oocyte of a black mouse with an injection pipette, activated the oocyte in a medium of strontium ions and cytochalasin B, and implanted it into an albino mouse foster mother. This technique differed from the way Dolly the sheep was created, which relied on electric shocks to fuse a mammary gland cell with an enucleated oocyte. Both methods sought to revert DNA taken from an adult animal to an embryonic stage. The success rate of the "Honolulu technique" was on the order of 2-3%.
- Yanagimachi's group continued the cloning process, creating multiple generations (clones of clones). Cumulina's ability to age and reproduce were of interest to the researchers. She died at an advanced age and produced two litters.
- References:
- Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature. 1998 Jul 23;394(6691):369-74. doi: 10.1038/28615. PMID: 9690471.
- Wakayama T, Yanagimachi R. Cloning the laboratory mouse. Semin Cell Dev Biol. 1999 Jun;10(3):253-8. doi: 10.1006/scdb.1998.0267. PMID: 10441536.
- Weiss, Rick. Scientists Clone Mice. Washington Post. 1998 July 23: page A01.
- Cohen, P. Cloning by numbers. New Scientist. 1998 Dec 19.
- CBSNews.com, First Cloned Mouse Dies of Old Age, 2000 May 10. https://www.cbsnews.com/news/first-cloned-mouse-dies-of-old-age/ (last accessed 2022/01/24)
- BBC News, First mouse clone dies, 2000 May 10. http://news.bbc.co.uk/2/hi/science/nature/743579.stm (last accessed 2022/01/24)
- Location
- Currently not on view
- Credit Line
- Gift of the University of Hawaii at Manoa
- 1997-10-03
- ID Number
- 2021.0088.01
- catalog number
- 2021.0088.01
- accession number
- 2021.0088
- Object Name
- mouse
- Measurements
- overall: 11 cm x 8 cm x 7 cm x 0 cm; 4 11/32 in x 3 5/32 in x 2 3/4 in x 0 in
- place made
- United States: Hawaii, Oahu, Honolulu
- See more items in
- Medicine and Science: Biological Sciences
- National Museum of American History
- Record ID
- nmah_2012238
- GUID (Link to Original Record)
- https://n2t.net/ark:/65665/ng456166f62-bc49-499a-8a4b-4fc11301e3ed
